Single-Molecule Tracking and Super-Resolution Microscopy Unveil Actin-Driven Membrane Nanotopography Shaping Stable Integrin Adhesions in Developing Tissue.
Tianchi Chen, Cecilia H Fernández‐Espartero, Grégory Giannone
Cytoskeleton. 2025-01-06
10.1002/cm.21970

Actin-driven nanotopography promotes stable integrin adhesion formation in developing tissue
Tianchi Chen, Cecilia H. Fernández-Espartero, Abigail Illand, Ching-Ting Tsai, Yang Yang, Benjamin Klapholz, Pierre Jouchet, Mélanie Fabre, Olivier Rossier, Bianxiao Cui, Sandrine Lévêque-Fort, Nicholas H. Brown, Grégory Giannone
Nat Commun. 2024-10-07
10.1038/s41467-024-52899-x

Single molecule imaging unveils cellular architecture, dynamics and mechanobiology.
Tianchi Chen, Grégory Giannone
Current Opinion in Cell Biology. 2024-06-01
10.1016/j.ceb.2024.102369

Actin-nucleation promoting factor N-WASP influences alpha-synuclein condensates and pathology.
Joshua Jackson, Christian Hoffmann, Enzo Scifo, Han Wang, Lena Wischhof, Antonia Piazzesi, Mrityunjoy Mondal, Hanna Shields, Xuesi Zhou, Magali Mondin, Eanna B. Ryan, Hermann Döring, Jochen H. M. Prehn, Klemens Rottner, Gregory Giannone, Pierluigi Nicotera, Dan Ehninger, Dragomir Milovanovic, Daniele Bano
Cell Death Dis. 2024-04-30
10.1038/s41419-024-06686-7

Synapsin condensation controls synaptic vesicle sequestering and dynamics.
Christian Hoffmann, Jakob Rentsch, Taka A. Tsunoyama, Akshita Chhabra, Gerard Aguilar Perez, Rajdeep Chowdhury, Franziska Trnka, Aleksandr A. Korobeinikov, Ali H. Shaib, Marcelo Ganzella, Gregory Giannone, Silvio O. Rizzoli, Akihiro Kusumi, Helge Ewers, Dragomir Milovanovic
Nat Commun. 2023-10-23
10.1038/s41467-023-42372-6

Compressive forces stabilize microtubules in living cells.
Yuhui Li, Ondřej Kučera, Damien Cuvelier, David M. Rutkowski, Mathieu Deygas, Dipti Rai, Tonja Pavlovič, Filipe Nunes Vicente, Matthieu Piel, Grégory Giannone, Dimitrios Vavylonis, Anna Akhmanova, Laurent Blanchoin, Manuel Théry
Nat. Mater.. 2023-06-29
10.1038/s41563-023-01578-1

Integrin-based adhesion compartmentalizes ALK3 of the BMPRII to control cell adhesion and migration
Amaris Guevara-Garcia, Laure Fourel, Ingrid Bourrin-Reynard, Adria Sales, Christiane Oddou, Mylène Pezet, Olivier Rossier, Paul Machillot, Line Chaar, Anne-Pascale Bouin, Gregory Giannone, Olivier Destaing, Catherine Picart, Corinne Albiges-Rizo
Journal of Cell Biology. 2022-10-07
10.1083/jcb.202107110

Talin and kindlin cooperate to control the density of integrin clusters
Julien Pernier, Marcelina Cardoso Dos Santos, Mariem Souissi, Adrien Joly, Hemalatha Narassimprakash, Olivier Rossier, Grégory Giannone, Emmanuèle Helfer, Kheya Sengupta, Christophe Le Clainche
. 2022-10-07
10.1101/2022.10.07.511266

Novel imaging methods and force probes for molecular mechanobiology of cytoskeleton and adhesion
Filipe Nunes Vicente, Tianchi Chen, Olivier Rossier, Grégory Giannone
Trends in Cell Biology. 2022-08-01
10.1016/j.tcb.2022.07.008

Molecular organization and mechanics of single vimentin filaments revealed by super-resolution imaging.
Filipe Nunes Vicente, Mickael Lelek, Jean-Yves Tinevez, Quang D. Tran, Gerard Pehau-Arnaudet, Christophe Zimmer, Sandrine Etienne-Manneville, Gregory Giannone, Cécile Leduc
Sci. Adv.. 2022-02-25
10.1126/sciadv.abm2696

Une fenêtre sur la mécano-biologie à l’échelle moléculaire grâce à la microscopie de super-résolution
Filipe Nunes Vicente, Olivier Rossier, Grégory Giannone
Med Sci (Paris). 2022-01-01
10.1051/medsci/2021233

Forces generated by lamellipodial actin filament elongation regulate the WAVE complex during cell migration
Amine Mehidi, Frieda Kage, Zeynep Karatas, Maureen Cercy, Matthias Schaks, Anna Polesskaya, Matthieu Sainlos, Alexis M. Gautreau, Olivier Rossier, Klemens Rottner, Grégory Giannone
Nat Cell Biol. 2021-11-01
10.1038/s41556-021-00786-8

Integrin-Functionalised Giant Unilamellar Vesicles via Gel-Assisted Formation: Good Practices and Pitfalls
Mariem Souissi, Julien Pernier, Olivier Rossier, Gregory Giannone, Christophe Le Clainche, Emmanuèle Helfer, Kheya Sengupta
IJMS. 2021-06-13
10.3390/ijms22126335

Molecular motion and tridimensional nanoscale localization of kindlin control integrin activation in focal adhesions.
Thomas Orré, Adrien Joly, Zeynep Karatas, Birgit Kastberger, Clément Cabriel, Ralph T. Böttcher, Sandrine Lévêque-Fort, Jean-Baptiste Sibarita, Reinhard Fässler, Bernhard Wehrle-Haller, Olivier Rossier, Grégory Giannone
Nat Commun. 2021-05-25
10.1038/s41467-021-23372-w

Engineering paralog-specific PSD-95 synthetic binders as potent and minimally invasive imaging probes
Charlotte Rimbault, Christelle Breillat, Benjamin Compans, Estelle Toulmé, Filipe Nunes Vicente, Monica Fernandez-Monreal, Patrice Mascalchi, Camille Genuer, Virginia Puente-Muñoz, Isabel Gauthereau, Eric Hosy, Gregory Giannone, Ingrid Chamma, Cameron D. Mackereth, Christel Poujol, Daniel Choquet, Matthieu Sainlos
. 2021-04-08
10.1101/2021.04.07.438431

Single-Protein Tracking to Study Protein Interactions During Integrin-Based Migration.
A. V. Radhakrishnan, Tianchi Chen, Jose Filipe Nunes Vicente, Thomas Orré, Amine Mehidi, Olivier Rossier, Grégory Giannone
The Integrin Interactome. 2020-11-20
10.1007/978-1-0716-0962-0_8

Cell stretching is amplified by active actin remodelling to deform and recruit proteins in mechanosensitive structures
Sophie Massou, Filipe Nunes Vicente, Franziska Wetzel, Amine Mehidi, Dan Strehle, Cecile Leduc, Raphaël Voituriez, Olivier Rossier, Pierre Nassoy, Grégory Giannone
Nat Cell Biol. 2020-07-27
10.1038/s41556-020-0548-2

A micromechanical cell stretching device compatible with super-resolution microscopy and single protein tracking
Filipe Nunes Vicente, Sophie Massou, Franziska Wetzel, Amine Mehidi, Dan Strehle, Cecile Leduc, Raphaël Voituriez, Olivier Rossier, Nassoy Pierre, Grégory Giannone
. 2020-07-27
10.21203/rs.3.pex-961/v1

Actin dynamics in cell migration.
Matthias Schaks, Grégory Giannone, Klemens Rottner
Essays in Biochemistry. 2019-09-24
10.1042/ebc20190015

Transient Activations of Rac1 at the Lamellipodium Tip Trigger Membrane Protrusion.
Amine Mehidi, Olivier Rossier, Matthias Schaks, Anaël Chazeau, Fabien Binamé, Amanda Remorino, Mathieu Coppey, Zeynep Karatas, Jean-Baptiste Sibarita, Klemens Rottner, Violaine Moreau, Grégory Giannone
Current Biology. 2019-09-01
10.1016/j.cub.2019.07.035

The inner life of integrin adhesion sites: From single molecules to functional macromolecular complexes.
Thomas Orré, Olivier Rossier, Grégory Giannone
Experimental Cell Research. 2019-06-01
10.1016/j.yexcr.2019.03.036

A tessellation-based colocalization analysis approach for single-molecule localization microscopy
Florian Levet, Guillaume Julien, Rémi Galland, Corey Butler, Anne Beghin, Anaël Chazeau, Philipp Hoess, Jonas Ries, Grégory Giannone, Jean-Baptiste Sibarita
Nat Commun. 2019-05-30
10.1038/s41467-019-10007-4

Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D
Jeanne Linarès-Loyez, Joana S. Ferreira, Olivier Rossier, Brahim Lounis, Gregory Giannone, Laurent Groc, Laurent Cognet, Pierre Bon
Front. Phys.. 2019-05-08
10.3389/fphy.2019.00068

Using Single-Protein Tracking to Study Cell Migration.
Thomas Orré, Amine Mehidi, Sophie Massou, Olivier Rossier, Grégory Giannone
Methods in Molecular Biology. 2018-01-01
10.1007/978-1-4939-7701-7_21

Optimized labeling of membrane proteins for applications to super-resolution imaging in confined cellular environments using monomeric streptavidin
Ingrid Chamma, Olivier Rossier, Grégory Giannone, Olivier Thoumine, Matthieu Sainlos
Nat Protoc. 2017-03-09
10.1038/nprot.2017.010

Organization and dynamics of the actin cytoskeleton during dendritic spine morphological remodeling.
Anaël Chazeau, Grégory Giannone
Cell. Mol. Life Sci.. 2016-04-22
10.1007/s00018-016-2214-1

Cytotoxic T Cells Use Mechanical Force to Potentiate Target Cell Killing.
Roshni Basu, Benjamin M. Whitlock, Julien Husson, Audrey Le Floc’h, Weiyang Jin, Alon Oyler-Yaniv, Farokh Dotiwala, Gregory Giannone, Claire Hivroz, Nicolas Biais, Judy Lieberman, Lance C. Kam, Morgan Huse
Cell. 2016-03-01
10.1016/j.cell.2016.01.021

Super-resolution links vinculin localization to function in focal adhesions.
Grégory Giannone
Nat Cell Biol. 2015-06-30
10.1038/ncb3196

Single-molecule imaging in live cell using gold nanoparticles.
Cécile Leduc, Satyabrata Si, Jérémie J. Gautier, Zhenghong Gao, Edakkattuparambil S. Shibu, Alexis Gautreau, Grégory Giannone, Laurent Cognet, Brahim Lounis
Methods in Cell Biology. 2015-01-01
10.1016/bs.mcb.2014.10.002

Nanoscale segregation of actin nucleation and elongation factors determines dendritic spine protrusion.
Anaël Chazeau, Amine Mehidi, Deepak Nair, Jérémie J Gautier, Cécile Leduc, Ingrid Chamma, Frieda Kage, Adel Kechkar, Olivier Thoumine, Klemens Rottner, Daniel Choquet, Alexis Gautreau, Jean‐Baptiste Sibarita, Grégory Giannone
EMBO J.. 2014-10-07
10.15252/embj.201488837

The cancer glycocalyx mechanically primes integrin-mediated growth and survival.
Matthew J. Paszek, Christopher C. DuFort, Olivier Rossier, Russell Bainer, Janna K. Mouw, Kamil Godula, Jason E. Hudak, Jonathon N. Lakins, Amanda C. Wijekoon, Luke Cassereau, Matthew G. Rubashkin, Mark J. Magbanua, Kurt S. Thorn, Michael W. Davidson, Hope S. Rugo, John W. Park, Daniel A. Hammer, Grégory Giannone, Carolyn R. Bertozzi, Valerie M. Weaver
Nature. 2014-06-25
10.1038/nature13535

Glutamate involvement in calcium-dependent migration of astrocytoma cells.
Abdelkader Hamadi, Grégory Giannone, Kenneth Takeda, Philippe Rondé
Cancer Cell International. 2014-01-01
10.1186/1475-2867-14-42

A highly specific gold nanoprobe for live-cell single-molecule imaging.
Cécile Leduc, Satyabrata Si, Jérémie Gautier, Martinho Soto-Ribeiro, Bernhard Wehrle-Haller, Alexis Gautreau, Grégory Giannone, Laurent Cognet, Brahim Lounis
Nano Lett.. 2013-03-06
10.1021/nl304561g

Quantum-yield-optimized fluorophores for site-specific labeling and super-resolution imaging.
Christian Grunwald, Katrin Schulze, Gregory Giannone, Laurent Cognet, Brahim Lounis, Daniel Choquet, Robert Tampé
J. Am. Chem. Soc.. 2011-06-01
10.1021/ja200967z

Multi-level molecular clutches in motile cell processes.
Grégory Giannone, René-Marc Mège, Olivier Thoumine
Trends in Cell Biology. 2009-09-01
10.1016/j.tcb.2009.07.001

Neurexin/neuroligin interaction kinetics characterized by counting single cell-surface attached quantum dots.
Edouard Saint-Michel, Grégory Giannone, Daniel Choquet, Olivier Thoumine
Biophysical Journal. 2009-07-01
10.1016/j.bpj.2009.04.044

Activity-independent and subunit-specific recruitment of functional AMPA receptors at neurexin/neuroligin contacts.
M. Heine, O. Thoumine, M. Mondin, B. Tessier, G. Giannone, D. Choquet
Proceedings of the National Academy of Sciences. 2008-12-19
10.1073/pnas.0804007106

Sensitive liposomes encoded with oligonucleotide amphiphiles: a biocompatible switch.
Arnaud Gissot, Carmelo Di Primo, Isabelle Bestel, Gregory Giannone, Hubert Chapuis, Philippe Barthélémy
Chem. Commun.. 2008-01-01
10.1039/b812398e

Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow.
Yunfei Cai, Nicolas Biais, Gregory Giannone, Monica Tanase, Guoying Jiang, Jake M. Hofman, Chris H. Wiggins, Pascal Silberzan, Axel Buguin, Benoit Ladoux, Michael P. Sheetz
Biophysical Journal. 2006-11-01
10.1529/biophysj.106.084806

Lateral membrane waves constitute a universal dynamic pattern of motile cells.
Hans-Günther Döbereiner, Benjamin J. Dubin-Thaler, Jake M. Hofman, Harry S. Xenias, Tasha N. Sims, Grégory Giannone, Michael L. Dustin, Chris H. Wiggins, Michael P. Sheetz
Phys. Rev. Lett.. 2006-07-20
10.1103/physrevlett.97.038102

Substrate rigidity and force define form through tyrosine phosphatase and kinase pathways.
Grégory Giannone, Michael P. Sheetz
Trends in Cell Biology. 2006-04-01
10.1016/j.tcb.2006.02.005

Force sensing and generation in cell phases: analyses of complex functions.
Hans-Günther Döbereiner, Benjamin J. Dubin-Thaler, Gregory Giannone, Michael P. Sheetz
Journal of Applied Physiology. 2005-04-01
10.1152/japplphysiol.01181.2004

Dynamic phase transitions in cell spreading.
Hans-Günther Döbereiner, Benjamin Dubin-Thaler, Grégory Giannone, Harry S. Xenias, Michael P. Sheetz
Phys. Rev. Lett.. 2004-09-02
10.1103/physrevlett.93.108105

Calcium rises locally trigger focal adhesion disassembly and enhance residency of focal adhesion kinase at focal adhesions.
Grégory Giannone, Philippe Rondé, Mireille Gaire, Joël Beaudouin, Jacques Haiech, Jan Ellenberg, Kenneth Takeda
J. Biol. Chem.. 2004-04-21
10.1074/jbc.m404054200

Nanometer analysis of cell spreading on matrix-coated surfaces reveals two distinct cell states and STEPs.
Benjamin J. Dubin-Thaler, Gregory Giannone, Hans-Günther Döbereiner, Michael P. Sheetz
Biophysical Journal. 2004-03-01
10.1016/s0006-3495(04)74246-0

Periodic lamellipodial contractions correlate with rearward actin waves.
Grégory Giannone, Benjamin J Dubin-Thaler, Hans-Günther Döbereiner, Nelly Kieffer, Anne R Bresnick, Michael P Sheetz
Cell. 2004-02-01
10.1016/s0092-8674(04)00058-3

Talin1 is critical for force-dependent reinforcement of initial integrin-cytoskeleton bonds but not tyrosine kinase activation.
Grégory Giannone, Guoying Jiang, Deborah H. Sutton, David R. Critchley, Michael P. Sheetz
J Cell Biol. 2003-10-27
10.1083/jcb.200302001

Delphinidin, an active compound of red wine, inhibits endothelial cell apoptosis via nitric oxide pathway and regulation of calcium homeostasis.
Sophie Martin, Grégory Giannone, Ramaroson Andriantsitohaina, M Carmen Martinez
British Journal of Pharmacology. 2003-07-01
10.1038/sj.bjp.0705347

Two-piconewton slip bond between fibronectin and the cytoskeleton depends on talin.
Guoying Jiang, Grégory Giannone, David R. Critchley, Emiko Fukumoto, Michael P. Sheetz
Nature. 2003-07-01
10.1038/nature01805

Calcium oscillations trigger focal adhesion disassembly in human U87 astrocytoma cells.
Gregory Giannone, Philippe Rondé, Mireille Gaire, Jacques Haiech, Kenneth Takeda
J. Biol. Chem.. 2002-05-14
10.1074/jbc.m203952200

Mechanism of calcium oscillations in migrating human astrocytoma cells.
Philippe Rondé, Gregory Giannone, Inna Gerasymova, Herrade Stoeckel, Kenneth Takeda, Jacques Haiech
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 2000-12-01
10.1016/s0167-4889(00)00102-6

Novel activation of non-selective cationic channels by dinitrosyl iron-thiosulfate in PC12 cells.
Grégory Giannone, Kenneth Takeda, Andrei L. Kleschyov
The Journal of Physiology. 2000-12-01
10.1111/j.1469-7793.2000.00735.x